Proprietary biomarker assays
Enzyme-Linked Immunoassay (ELISA)
The ELISA sphingotest® penKid® represents a product expansion of SphingoTec’s reference assay technology and enables the quantitative measurement of Proenkephalin A 119–159 (penKid). The assay was developed to closely match the performance of SphingoTec’s chemiluminescence reference assay while allowing use on standard laboratory photometers. This format enables straightforward implementation in clinical and research laboratories and provides investigators with reliable access to penKid measurement for scientific and translational studies.
ELISA sphingotest® penKid®
Enzyme-linked immunosorbent assay for the quantification of human Proenkephalin A 119-159 in plasma.
Key features
| ELISA sphingotest® penKid® | |
|---|---|
| Assay type | Enzyme-linked Immunosorbent Assay (ELISA) |
| Assay format | One-step |
| Plate type | 1x96 well plate |
| Product status | RUO |
| Sample type | EDTA plasma, heparin plasma |
| Sample Volume | 50 µL |
| Number of Calibrators | 1 vial (6 levels generated by the customer) |
| Measuring Unit | pmol/L |
| Measuring Range | 10 - 1,000 pmol/L |
| Assay incubation time | 2.5 hours |
| Lower Limit of Quantification (LoQ) | 10.2 pmol/L (20% Total CV) |
| Lower Limit of Detection (LoD) | 8.9 pmol/L |
| High Dose Hook | No high-dose hook effect up to 10.000 pmol/L |
| Detection Method | Colorimetry (detection wavelenght = 450 nm, reference wavelenght = 570 nm) |
| Storage Condition | 2-8°C |
Immunoluminometric Assay
SphingoTec’s reference assays are based on acridinium ester chemiluminescence sandwich immunoassay technology and enable the quantitative measurement of the proprietary biomarkersProenkephalin A 119–159 (penKid) and bioactive Adrenomedullin 1–52 (bio-ADM). This technology has been used to analyze more than 100,000 clinical samples across more than 200 studies and serves as the analytical reference standard for calibration and correlation of licensed assays such as AFIAS sphingotest® penKid® and product expansions including the ELISA sphingotest® penKid®.
EDTA plasma
sphingotest® microtiter plate assay requires EDTA plasma
High throughput
96 well plate test kit contains enough reagent to run 41 samples in double determinations
1h incubation
Following a 30-minute hands-on time, the assay incubates for 1 hour
sphingotest® penKid®
Immunoluminometric assay (ILMA) for the determination of Proenkephalin A 119 - 159 (penKid) in human EDTA plasma.
Key features
| sphingotest® penKid® | |
|---|---|
| Assay type | Immunoluminometric Assay (ILMA) |
| Assay format | One-step |
| Plate type | 12 x 8 well plate |
| Product status | RUO |
| Number of calibrators | 5 |
| Number of controls | 2 |
| Sample type | EDTA plasma |
| Sample volume | 50 µL |
| Assay incubation time | 1 hour |
| Measuring unit | pmol/L |
| Measuring range | 30 - 1,172 pmol/L |
| Lower Limit of Quantification (LoQ) | 29.9 pmol/L |
| Lower Limit of Detection (LoD) | 29.9 pmol/L |
| High Dose Hook | No high-dose hook effect up to 10.000 pmo/L |
| Detection method | Flash-type chemiluminescence (acridinium ester) |
| Storage condition | 2-8°C |
sphingotest® bio-ADM®
Immunoluminometric assay (ILMA) for the determination of bioactive Adrenomedullin 1 - 52 (bio-ADM) in human EDTA plasma.
Key features
| sphingotest® bio-ADM® | |
|---|---|
| Assay type | Immunoluminometric Assay (ILMA) |
| Assay format | One-step |
| Plate type | 12 x 8 well plate |
| Product status | RUO |
| Number of calibrators | 5 |
| Number of controls | 2 |
| Sample type | EDTA plasma |
| Sample volume | 50 µL |
| Assay incubation time | 1 hour |
| Measuring unit | pg/mL |
| Measuring range | 11 - 1,094 pg/mL |
| Lower Limit of Quantification (LoQ) | 10.8 pg/mL (20% Total CV) |
| Lower Limit of Detection (LoD) | 6.3 pg/mL |
| High Dose Hook | No high-dose hook effect up to 10 µg/mL |
| Detection method | Flash-type chemiluminescence (acridinium ester) |
| Storage condition | 2-8°C |
Special material required but not provided:
sphingotest® Lightning
Instructions for useThe sphingotest® Lightning is a reagent intended for research use only and used for automated chemiluminescence measurement of sphingotest® immunoluminometric assays (ILMA) using acridinium ester technology in conjunction with an automated flash-type luminometer.
MTP flash-type luminometer with two injectors
MTP flash-type luminometer compatible software and validation tool
*Disclaimer
ELISA sphingotest® penKid®, sphingotest® penKid®, sphingotest® bio-ADM® are offered for research use only. “penKid” and “bio-ADM” represent the analytes Proenkephalin A 119-159, and bioactive Adrenomedullin 1-52 respectively.