Microtiter plate assays (MTP)
Immunoassay for research purposes
The microtiter plate is a standard tool in analytical research and diagnostic testing laboratories. SphingoTec’s microtiter plate assays are based on a chemiluminescence sandwich immunoassay technology and allow the measurement of SphingoTec’s proprietary biomarkers Proenkephalin A 119-159 (penKid) and bioactive Adrenomedullin 1-52 (bio-ADM).
EDTA plasma
sphingotest® microtiter plate assay requires EDTA plasma
High throughput
96 well plate test kit contains enough reagent to run 41 samples in double determinations
1h incubation
Following a 30-minute hands-on time, the assay incubates for 1 hour
sphingotest® penKid®
Immunoluminometric assay (ILMA) for the determination of Proenkephalin A 119 - 159 (penKid) in human EDTA plasma.
sphingotest® bio-ADM®
Immunoluminometric assay (ILMA) for the determination of bioactive Adrenomedullin 1 - 52 (bio-ADM) in human EDTA plasma.
Key features
| sphingotest® penKid® | |
|---|---|
| Product type | non-automated immunoluminometric assay (ILMA) |
| Product status | RUO |
| Sample type | EDTA plasma samples |
| Samples volume | 50 µL |
| Kit size | 1 x 96 well plate |
| Assay incubation time | approx. 1 hour |
| Detection range | 30 - 1,172 pmol/L |
| Upper normal range | 89 pmol/L (90% CI 85–118 pmol/L) |
| No high dose hook effect | up to 100,000 pmol/L |
| sphingotest® bio-ADM® | |
|---|---|
| Product type | non-automated immunoluminometric assay (ILMA) |
| Product status | RUO |
| Sample type | EDTA plasma samples |
| Samples volume | 100 µL |
| Kit size | 1 x 96 well plate |
| Assay incubation time | approx. 1 hour |
| Detection range | 11 - 1094 pg/mL |
| Upper normal range | 29 pg/mL (90% CI 27 pg/mL - 38 pg/mL) |
| No high dose hook effect | up to 10 µg/mL |
Special material required but not provided:
sphingotest® Lightning
The sphingotest® Lightning is a reagent intended for research use only and used for automated chemiluminescence measurement of sphingotest® immunoluminometric assays (ILMA) using acridinium ester technology in conjunction with an automated flash-type luminometer.
Product Instruction for Use is available upon request.
MTP flash-type luminometer with two injectors
MTP flash-type luminometer compatible software and validation tool
*Disclaimer
Sphingotest® penKid®, sphingotest® bio-ADM® are offered for research use only. “penKid” and “bio-ADM” represent the analytes Proenkephalin A 119-159, and bioactive Adrenomedullin 1-52 respectively.