sphingotest® bio-ADM®
€1.995,00
Immunoluminometric assay for the quantification of bioactive Adrenomedullin 1-52 in human EDTA plasma.
Intended Use
The sphingotest® bio-ADM® is a non-automated immunoluminometric assay (ILMA) for the quantitative measurement of bioactive Adrenomedullin 1-52 in human EDTA plasma. The sphingotest® bio-ADM® is intended for use by professional users in laboratory settings. The product is for research use only. It is not intended for diagnostic or therapeutic purpose.
SPECIFICATIONS
Assay type: Immunoluminometric Assay (ILMA)
Assay format: One-step
Plate type: 12 x 8 well plate
Product status: RUO
Number of calibrators: 5
Number of controls: 2
Sample type: EDTA plasma
Sample volume: 50 µL
Assay incubation time: 1 hour
Measuring unit: pg/mL
Measuring range: 11 - 1,094 pg/mL
Lower Limit of Quantification (LoQ): 10.8 pg/mL (20% Total CV)
Lower Limit of Detection (LoD): 6.3 pg/mL
High Dose Hook: No high-dose hook effect up to 10 µg/mL
Detection method: Flash-type chemiluminescence (acridinium ester)
Storage condition: 2-8°C
USAGE
DETAILS
- CALIBRATOR: 5 vials, lyophilized for 1 mL (CAL1 to CAL5)
- CONTROL: 2 vials, lyophilized for 1 mL (CON A and CON B)
- ZERO MATRIX: 1 bottle, 8 mL, liquid, for reconstitution of CALIBRATOR and CONTROLS
- MICROPLATE: 1 pouched 96-well microplate, coated with anti-bio-ADM monoclonal antibody
- TRACER: 1 bottle, lyophilized for 18 mL, contains acridinium-ester-labelled anti-bio-ADM monoclonal antibody
- BUFFER: 1 bottle, 18 mL, liquid, for reconstitution of TRACER
- WASH CONCENTRATE: 1 bottle, 22 mL concentrate, liquid, for generation of 1.1 L wash solution
- ADHESIVE FOIL: 1 black colored adhesive foil, for light protected incubation
- IFU: 1 Instruction for use
- QR: 1 Quality report with lot numbers and calibration info
Reagents and materials required but not provided
- Microlitre pipettes (100 μL, 150 μL, 1000 µL) with replaceable plastic tip
- Multichannel pipette (150 µL) with replaceable plastic tip
- Vortex mixer
- Laboratory water grade 2 (acc. ISO 3696:1987)
- Bottle with lid or screw cap (1,100 mL) to fit the ready-to-use wash solution
- sphingotest® Lightning (cat. No. 080-04000): contains reagents sufficient for up to 3,000 single luminescence measurements; contains controls for daily system suitability test of the MTP luminometer.
- Horizontal shaker with 600 rpm and 3 mm shaking stroke (e.g., Titramax 101, Heidolph)
- Automatic MTP washing device (e.g., Wellwash, Thermo Scientific)
- MTP luminometer with two injectors, each of 100 µL injection volume (e.g., Centro LB 960 or Centro LB 963, Berthold Technologies GmbH & Co. KG, Wildbad, Germa-ny)Software and validation tool compatible with the MTP luminometer (e.g., Instrument Control and Evaluation, Berthold Technologies GmbH & Co. KG, Wildbad, Germany)
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