sphingotest® penKid®

€1.050,00

• SPECIFICATIONS

  Assay type: Immunoluminometric Assay (ILMA)

  Assay format: One-step

  Plate type: 12 x 8 well plate

  Product status: RUO

  Number of calibrators: 5

  Number of controls: 2

  Sample type: EDTA plasma

  Sample volume: 50 µL

  Assay incubation time: 1 hour

  Measuring unit: pmol/L

  Measuring range: 30 - 1,172 pmol/L

  Lower Limit of Quantification (LoQ): 29.9 pmol/L

  Lower Limit of Detection (LoD): 29.9 pmol/L

  High Dose Hook: No high-dose hook effect up to 10.000 pmo/L

  Detection method: Flash-type chemiluminescence (acridinium ester)

  Storage condition: 2-8°C

• USAGE

  Instructions for use

• DETAILS

  • CALIBRATOR: 5 vials, lyophilizedfor 0.5 mL (CAL1 to CAL5)
  • CONTROL: 2 vials, lyophilized for 0.5 mL (CON A and CON B)
  • ZERO MATRIX: 1 bottle, 4 mL, liquid, for reconstitution of CALIBRATOR and CONTROLS
  • MICROPLATE: 1 pouched 96-well microplate, coated with anti-penKid monoclonal antibody
  • TRACER: 1 bottle, lyophilized for 18 mL, contains acridinium-ester-labelled anti-penKid monoclonal antibody
  • BUFFER: 1 bottle, 18 mL, liquid, for reconstitution of TRACER
  • WASH CONCENTRATE: 1 bottle, 22 mL concentrate, liquid, for generation of 1.1 L wash solution
  • ADHESIVE FOIL: 1 black colored adhesive foil, for light protected incubation
  • IFU: 1 Instruction for use
  • QR: 1 Quality report with lot numbers and calibration info

  
  

  Reagents and materials required but not provided

  
  

  • Microlitre pipettes (50 μL, 150 μL, 500 µL) with replaceable plastic tip
  • Multichannel pipette (150 µL) with replaceable plastic tip
  • Vortex mixer
  • Laboratory water grade 2 (acc. ISO 3696:1987)
  • Bottle with lid or screw cap (1,100 mL) to fit the ready-to-use wash solution
  • sphingotest® Lightning (cat. No. 080-04000): contains reagents sufficient for up to 3,000 single luminescence measurements; contains controls for daily system suitability test of the MTP luminometer
  • Automated MTP washing device (e.g. Wellwash, Thermo Scientific)
  • MTP luminometer with two injectors, each of 100 µL injection volume (e.g. Centro LB 963, Berthold Technologies GmbH & Co. KG, Wildbad, Germany)
  • Software and validation tool compatible with the MTP luminometer (e.g. Instrument Con-trol and Evaluation, Berthold Technologies GmbH & Co. KG, Wildbad, Germany)