ELISA sphingotest® penKid®

€675,00

• SPECIFICATIONS

  Assay type:  Enzyme-linked Immunosorbent Assay (ELISA)

  Assay format: One-step

  Plate type: 1 x 96 well plate

  Product status: RUO

  Sample type: EDTA plasma, heparin plasma

  Sample Volume: 50 µL

  Number of Calibrators: 1 vial (6 levels generated by the customer)

  Measuring Unit: pmol/L

  Measuring Range: 10 – 1,000 pmol/L

  Assay incubation time: 2.5 hours

  Lower Limit of Quantification (LoQ): 10.2 pmol/L (20% Total CV)

  Lower Limit of Detection (LoD): 8.9 pmol/L

  High Dose Hook: No high-dose hook effect up to 10.000 pmol/L

  Detection Method: Colorimetry (detection wavelength = 450 nm, reference wavelength = 570 nm)

  Storage Condition: 2-8°C

• USAGE

  Instructions for use

• DETAILS

  • CALIBRATOR: 1 vial, lyophilized for 0.5 mL, for generation of calibrator set (CAL1 to CAL5)
  • ZERO MATRIX: 1 bottle, 4 mL, liquid, for reconstitution of CALIBRATOR and its dilution, for use as zero calibrator (CAL0)
  • MICROPLATE: 1 pouched 96-well microplate, coated with anti-penKid monoclonal antibodies
  • TRACER: 1 bottle, lyophilized for 18 mL, contains HRP-labelled anti-penKid monoclonal antibody
  • BUFFER: 1 bottle, 18 mL, liquid, for reconstitution of TRACER
  • SUBSTRATE: 1 bottle, 11 mL, liquid, for HRP catalyzed TMB reaction
  • STOP SOLUTION: 1 bottle, 6 mL, liquid, for stop of substrate reaction
  • WASH CONCENTRATE: 1 bottle, 22 mL concentrate, liquid, for generation of 1.1 L wash solution
  • ADHESIVE FOIL: 2 black colored adhesive foils, for light protected incubations
  • PACKAGE INSERT: 1 Package Insert with instructions for use
  • QUALITY REPORT: 1 Quality report with lot numbers and calibration info

  
  

  Reagents and materials required but not provided

  
  

  • Microlitre pipettes covering a range of 50 – 500 µL with replace¬able plastic tip.
  • Multichannel pipette (150 µL) with replaceable plastic tip.
  • Polypropylene tubes (≥500 µL).
  • Vortex mixer.
  • Reagent reservoirs with capacity ≥18 mL (for pipetting tracer, substrate and stop solutions using a multichannel pipette).
  • Laboratory water grade 2 (acc. ISO 3696:1987).
  • Bottle with lid or screw cap (1,100 mL) to fit the ready-to-use wash solution
  • Automated microplate washing device (e.g. Wellwash, Thermo Scientific).
  • Microplate reader capable of reading absorbances at 450 nm and 570 nm (e.g. Spectra-Max ID5, Molecular Devices LLC).